Nitrate concentration and 15N signal of Streamwater and Precipitation from Archived Samples: Watershed 3
Nitrate concentration and 15N signal of Streamwater and Precipitation from Archived Samples: Watershed 3
Susana Bernal
Center for Advanced Studies of Blanes, Consejo Superior de Investigaciones Cientificas (CEAB-CSIC)
17300 Blanes
Lars Hedin
Princeton University
Department of Ecology and Evolutionary Biology
Princeton, NJ 08542
Gene E. Likens
Institute of Ecosystem Studies
2801 Sharon Turnpike
Millbrook, NY 12545
Stefan Gerber
University of Florida
Soil and Water Science Department
Gainesville, FL 32611
Don Buso
Institute of Ecosystem Studies
234 Mirror Lake Road
North Woodstock, NH 03262
This data set includes the analysis of 18 Oxygen isotopes in precipitation and streamwater archived samples from watershed 3.
KEYWORD SET: Hubbard Brook Ecosystem Study LTER
HBEF Watershed 3, HBR, Hubbard Brook LTER, isotope, nitrate, stream, watershed.
KEYWORD SET: LTER Core Research Areas
inorganic nutrients.
HBEF watershed 3 (WS3)
West bounding coordinate: -71.725029
East bounding coordinate: -71.716533
North bounding coordinate: 43.962196
South bounding coordinate: 43.954650
Stream water and bulk deposition samples from the different weirs and meteorological stations at HBEF have been stored at the US Department of Agriculture, Forest Service headquarters from as early as 1963. These archived samples have been kept at room temperature after adding reagent-grade chloroform for preservation (3) (1 mL L−1). We had access to archived samples from 1982 to 2007 collected at the weir of W3 (the hydrological reference watershed at HBEF) and bulk de-position samples from the rain gauge station 1 (43d57mN, 71d44mW).
All water samples were analyzed for nitrate with a Dionex ionic chromatograph, and the δ15N-NO3− isotopic signatures were analyzed using the denitrifier method. To minimize the influence of any storage effects on the isotopic signature of nitrate, we considered only archived samples for which there was no evidence that nitrate levels had changed significantly since they were first measured. Our criterion was fulfilled only in 19 of 100 samples and comprised 11 stream water samples and 8 bulk deposition samples from winter and early spring (January to April) for the period of 1990–2007
  • Bernal, S., Hedin, L.O., Likens, G.E., Gerber, S., and Buso, D.C.. 2012. Complex response of the forest nitrogen cycle to climate change. Proceedings of the National Academy of Sciences of the United States of America 109(9):3406-3411.
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The re-use of scientific data has the potential to greatly increase communication, collaboration and synthesis within and among disciplines, and thus is fostered, supported and encouraged. Permission to use this dataset is granted to the Data User free of charge subject to the following terms:

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2) Redistribution. The data and metadata are provided for use by the Data User. The Data User will not redistribute the original Data Set or metadata to others without the explicit permission of the Principal Investigator.

3) Citation. It is considered a matter of professional ethics to acknowledge the work of other scientists. Thus, the Data User will properly attribute the Data Set in any publications or in the metadata of any derived data products that were produced using the Data Set. Citation should take the following general form: Creator, Year of Data Publication, Title of Dataset, Publisher, Dataset identifier.

Citation example: Holmes, R.T. 2012. Bird Abundances at Hubbard Brook (1969-2010) and on three replicate plots (1986-2000) in the White Mountain National Forest. Durham, NH. Hubbard Brook Data Archive [Database]. (23 July 2012)

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Acknowledgment example: Data on [topic] were provided by [name of PI] on [date]. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. Significant funding for collection of these data was provided by [agency]-[grant number], [agency]-[grant number], etc.

5) Consultation and questions. Data users are strongly encouraged to consult with the Principal Investigator(s) who collected these data for further information. Also, when appropriate, Data Users should consider including the Principal Investigator as a collaborator and/or co-author in the use of these data.

6) Notification. The Data User will notify the Principal Investigator of any publication or derivative work based on the Data Set. The Data User will also provide the Principal Investigator and/or the administrator of the Hubbard Brook Ecosystem Study with a pdf or two reprints of any publication(s) resulting from use of the Data Set.

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Information Manager, Hubbard Brook LTER
234 Mirror Lake Road
North Woodstock, NH 03262

Phone: (603) 726-8902

Data file: W3stm_precip_no3.txt
Description: Archived stream and bulk precipitation data for Watershed 3 at Hubbard Brook LTER
Notes on Data: These samples are stored in the Sample Archive Building at the Robert S. Pierce Ecosystem Laboratory, Hubbard Brook Experimental Forest, North Woodstock, NH.
ColumnVariableDescriptionUnitsCoded?Missing value label
1SiteSample locationnoneynone
2SampleDateDate of sample collectionYYYY-MM-DDnnone
3TypeType of sample analysednoneynone
4NO3ppbnitrate concentration in ppb N-NO3 partPerBillionnnone
5delta_15N-NO3delta 15 N in nitrate measured in parts per thousand (o/oo)perMilnnone


Variable: Site
Watershed 3
Rain Gage 1
Variable: Type
Streamwater sample
Bulk precipitation sample